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Macrophage migration inhibitory factor assembles as a trimer composed of three identical subunits
Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa)
Source Mouse Synonym Mouse Tumor Necrosis Factor Receptor Superfamily
and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Hypoxia Inducible Factor 1 Alpha (HIF1a)
Magnetic Bead-Based Immunoprecipitation (IP) Kit IHC Kits Macrophage migration inhibitory factor assemblesProduct Specification Application IP, Co IP Physical Appearance Liquid Stability & Storage 12 months from date of receipt, 2 to 8 C as supplied Background Immunoprecipitation (IP) is a technique based on the specific antigen antibody binding reaction. The magnetic beads in this kit are covalently conjugated with protein A G, which can specifically bind to the Fc region of immunoglobulins (IgG) from various species. The workflow involves the following